HisTrap HP His tag protein purification columns
High-performance immobilized metal affinity chromatography (IMAC) columns for His-tag recombinant protein purification.
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
High resolution: due to small bead size (average bead size is 34 μm)
High binding capacity: at least 40 mg His tag protein per mL resin, for high yields
Negligible nickel leakage: helps retain activity and minimize protein precipitation
Broad reagent compatibility: suitable for use with a wide range of reducing agents, detergents, denaturants, and other additives
Convenient HiTrap format: 1 mL and 5 mL columns compatible with syringe, pump, or chromatography systems such as ?KTA or other FPLC systems
Ni Sepharose High Performance columns
His tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
Parameter | HisTrap HP, 5 x 1 ml |
---|---|
Chromatography technique | Histidine-tagged protein purification |
Bed dimensions | 7 × 25 mm |
Bed height | 25 mm |
Bed volume | 1 ml |
Binding Capacity/Column1 | At least 40 mg histidine-tagged protein/ml medium |
Column i.d. | 7 mm |
Flow rate2 | 4 ml/min (1 ml), 5 ml/min (5ml) |
Licensing | For licensing information, see the Licensing Statements page in the About Us section. |
Pressure max. (over the packed bed during operation) | 5 bar [0.5 MPa] (70 psi) |
Storage | 4 to 30°C, 20% Ethanol |
Pack size | 5 × 1 mL |