ELISpotPLUS for enumeration of cells secreting IFN-γ
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting mouse IFN-γ. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate.
Pre-coated plates reduce assay time and minimize variability.
Mouse IFN-γ ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.
Analyte Information
IFN-γ
Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
APPLICATION:
ELISpot
REACTIVITY:
Mouse
DOCUMENTATION:Datasheet/Protocol
SDS
CONTENTS:
Biotinylated detection mAb (R4-6A2)
Streptavidin-ALP
Substrate (BCIP/NBT-plus)
2 Pre-coated MSIP clear plates (mAb AN18)
SIZE:2 plates